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Image Search Results
Journal: PLoS ONE
Article Title: Laterally Orienting C. elegans Using Geometry at Microscale for High-Throughput Visual Screens in Neurodegeneration and Neuronal Development Studies
doi: 10.1371/journal.pone.0035037
Figure Lengend Snippet: (A) Zoomed in view of imaging area shown by dashed red box from . White arrow indicates device radius of curvature (RoC) from arc center to outer edge. Dashed line indicates length (L) between loading and imaging valves. Scale bar is 200 µm. (B) Frequency of lateral nematode orientation for various channel geometries with standard error of proportion. Triple asterisk indicates statistical significance compared to straight channel designs ( p <0.001 determined using chi-squared test). S represents straight channel, while remaining labels indicate the 105 µm, 125 µm, and 145 µm RoC devices respectively. L for all devices is 700 µm. (C) Nematode oriented laterally in curved channel device (both nerve cords visible). Commissures present on different focal plane are obscured. (D) 3-D model of animal body section and microscope objective (viewpoint reference) showing nerve cord placement for a laterally oriented animal. (E) Nematode in a non-lateral body orientation as observed when loading animals into straight channel devices; DNC not visible due to animal orientation. Animal is within the same field of view as seen in panel C. Arrowheads for images (C) and (E) indicate ventral nerve cord (VNC) determined by placement of VD and DD motor neuron cell bodies. Arrow indicates dorsal nerve cord (DNC). Scale bars are 100 µm. Transgene marker for all fluorescent images is juIs76(Punc-25::GFP ). (F) 3-D model of animal body section and microscope objective for non-lateral oriented animal. Model diagrams (D) and (F) not drawn to scale. Red lines illustrate dorso-ventral axis. Green spheres represent DD and VD neuron cell bodies.
Article Snippet: Animals were visualized with 20×/0.5NA, and 40×/0.75NA magnification objectives on a
Techniques: Imaging, Microscopy, Marker, Animal Model